Journal: Nature Communications

Article Title: Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration

doi: 10.1038/s41467-022-35183-8

Figure Lengend Snippet: a , b Immunofluorescence for desmin ( a ) and MYH7 ( b ) to determine the myofiber maturation and their quantification by fluorescence area. c Immunofluorescence for PAX7 to determine the population of muscle resident cells and their quantification by fluorescence area. d and e Immunofluorescence for CD31 ( d ) and α-SMA ( e ) and to evaluate angiogenesis. α-SMA positive arterioles and CD31 positive capillaries are quantified by their fluorescence area. f and g Immunofluorescence for neurofilament (NF) ( f ) and βIIIT ( g ) to observe the neural integration. Neurofilament amount and βIIIT magnitude are quantified by their fluorescence area. h-i , Immunofluorescence for CD68 (macrophage) ( h ) and CD3 (T-cells) ( i ) to explore the MSCs immunomodulatory effects in muscle regeneration. The signal intensity of CD68 and CD3 were quantified by their fluorescence area. Scale bar, 100 µm ( a , b , e ), 40 µm ( c , d , h , i ), 20 µm ( f , g ). All data are represented as mean ± SD. n = 6 per group at each time point. For each data, at least six imaging windows are randomly selected. Significant difference is determined by one-way ANOVA, followed by Tukey’s test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Then the sides were incubated at 4 °C for 24 h with rabbit anti-desmin (1:500, Servicebio, GB11081), rabbit anti-MYH7 (1:400, Servicebio, GB111857), mouse anti-human/mouse/rat/chicken Pax7 (5 µg/mL; R&D Systems, MAB1675), rabbit anti-CD31 (1:200, Servicebio, GB11063-3), rabbit anti-α-SMA (1:100, Servicebio, GB111364), rabbit anti-NF (1:100, Abcam, ab223343), rabbit anti-βIIIT (1:100, Abcam, ab229590), rat anti-CD68 (1:100, Invitrogen, Cat# 14-0681-82), rabbit anti-CD3 (1:100, Abcam, ab16669), rabbit anti-Ki67 (1:600, Servicebio, GB111141), rabbit anti-HLA (1:100, Abcam, ab52922).

Techniques: Immunofluorescence, Fluorescence, Imaging

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: Long-term CS exposure led to emphysema and skeletal muscle dysfunction. ( A – F ) Airway resistance (RL), lung compliance, FVC, FEV0.1, FEV0.1/FVC, and FEF25-75% were measured in mice; ( G ) H&E staining of lung tissues from mice exposed to air and CS; Arrows (left) indicate destruction of the alveolar wall; Arrow (right) indicate Inflammatory cells infiltrates in the lung parenchyma and interstitial spaces; The scale in each image is 100 μm; ( H and I ) histogram of MLI and DI; ( J ) FACS showed the neutrophils (CD11B + Ly6G + ) and eosinophils (CD11B + SiglecF + ) in BALF; ( K ) histogram of grip strength; ( L ) H&E staining of muscles from mice exposed to air and CS; Arrows indicate changes in muscle fibers and widening of gaps; The scale in each image is 100 μm; ( M ) WB showed the protein expression of Slow MyHC, Fast MyHC, MuRF1, Atrogin1 and MSTN in muscles of air and CS-exposed mice; ( N ) the expression of Slow MyHC in muscles from mice. n = 5, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The primary antibodies included anti-slow skeletal myosin heavy chain (MyHC) primary antibody (GB111858, Servicebio, Wuhan, China), anti-4 hydroxynonenal (4-HNE) primary antibody (ab46545, Abcam, Cambridge, UK), anti-GPX4 primary antibody (AF7020, Beyotime, Shanghai, China) and DAPI (Beyotime, Shanghai, China).

Techniques: Staining, Muscles, Expressing